BLUelf Protein Ladder

$125.00$850.00

Product Name Item# Size
BLUelf Prestained Protein ladder
$125.00
PM008-0500 1x500ul
BLUelf Prestained Protein ladder
$345.00
PM008-0500K 3x500ul
BLUelf Prestained Protein ladder
$630.00
PM008-0500M 6x500ul
BLUelf Prestained Protein ladder
$850.00
PM008-0500G 10x500ul
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Product Description
The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. All products are for research use only.

Specifications

• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

• 2~3 μl per well for general Western transferring.

• Apply more for thicker (> 1.5 mm) or larger gel.

Contents:

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.

Product Details

Product Name Item# Size
BLUelf Prestained Protein ladder
PM008-0500 1x500ul
BLUelf Prestained Protein ladder
PM008-0500K 3x500ul
BLUelf Prestained Protein ladder
PM008-0500M 6x500ul
BLUelf Prestained Protein ladder
PM008-0500G 10x500ul

Packaging

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Compliance

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Literature & Tools

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Applications

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